An analytical probe for (1-3)-beta-D-glucans (callose). Highly specific for (1-3)-beta-glucans [Evans et al. (1984) Carbohyd. Polymers4: 215-230; Stone et al. (1984) Protoplasma 122: 191-195]. May be used for the quantitative determination of callose [Kauss (1989) Plant Physiol.81: 171-176] and in fluorescence assays for (1-3)-beta-glucan synthase products.
Specific probes for the detection of AGPs in tissue sections [Anderson et al. (1977) Aust J. Plant Physiol. 4: 143-158]. May be used to detect and quantify AGPs in tissue extracts [Van Holst & Clarke (1985) Anal Biochem. 148: 446-450] and to detect AGPs in crossed-electrophoretic separations [Van Holst & Clarke (1986) Plant Physiol.80: 786-798].
Specifically hydrolyses beta-D-glucans containing both (1-3)- and (1-4)-beta-D-glucosidic linkages in linear sequences. Does not hydrolyse (1-4)-beta-D-glucans or (1-3)-beta-D-glucans [Anderson & Stone (1975) FEBS Letters52: 202-207].
Useful as a positive control in fluoroescence microscopy studies on callose using the aniline blue fluorochrome or (1-3)-beta-D-glucan specific monoclonal antibody.
Chemistry and Biology of (1-3)-beta-glucans
By Bruce A Stone and Adrienne E Clarke
Glucans, with the (1-3)-beta-glucosidic linkage as a major feature, are present in most higher plants and many lower plants and microorganisms. They may occur as major structural or storage components or be formed at very specific sites in response to particular developmental events or stimuli. In many cases their functional role is a mystery, in others it is well established. Their distribution and physiological involvement indicates that they are important to fields such as agriculture and biotechnology, and may also have impact in medicine, throught their role in immunology and cancer therapy.
Structural characterisation and physical chemistry of:
Enzyme depolymerising (1-3)-beta-glucans
Biosynthesis of (1-3)-beta-glucans
Fungal, yeast and lichen (1-3)-beta-glucans
Chemistry and physiology of higher plants (1-3)-beta-glucans (callose)
(1-3)(1-4)-beta-glucans in higher plants
(1-3)-beta-glucans in plant host-pathogen interactions
(1-3)-beta-glucans and (1-3)-glucan hydrolases in animals
(1-3)-beta-glucans and animal defence mechanisms
References and index
The book is an invaluable reference handbook not only for graduate students and teachers in universities but also for scientists in industrial, agricultural and medical research and develpment laboratories.
Special features of ‘Chemistry and Biology of (1-3)-beta-glucans’ include:
Hard cover & section sewn binding
Over 150 tables and illustrations
Exhaustive index of over 50 pages
First published 1992
USD$91 plus postage and handling
Chemistry, Biochemistry, and Biology of (1-3)-beta-Glucans and Related Polysaccharides
By Antony Bacic, Geoffrey B. Fincher, Bruce A. Stone
Topics of medical relevance include detailing the glucans’ interactions with the immune system and research for cancer therapy applications
Web resource links allow scientists to explore additional beta glucan research
Separate indexes divided into Species and Subject for enhanced searchability
The book presents a comprehensive, systematic and authoritative survey of information about a family of chemically related, but functionally diverse, naturally occurring polysaccharides- the (1-3)-glucans. International contributors describe the chemical and physicochemical properties of these glucans and their derivatives and the molecular biological and structural aspects of the enzymes involved in their formation and breakdown. A detailed analysis of their physiological roles in the various biological situations in which they are found will be provided. Additionally, evolutionary relationships among the family of these glucans will be described.