Aniline Blue Fluorochrome for callose and related (1-3)-beta-glucans
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An analytical probe for (1-3)-beta-D-glucans (callose). Highly specific for (1-3)-beta-glucans [Evans et al. (1984) Carbohyd. Polymers 4: 215-230; Stone et al. (1984) Protoplasma 122: 191-195]. May be used for the quantitative determination of callose [Kauss (1989) Plant Physiol. 81: 171-176] and in fluorescence assays for (1-3)-beta-glucan synthase products.
Yariv Reagents for arabinogalactan-proteins (AGPs)
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Specific probes for the detection of AGPs in tissue sections [Anderson et al. (1977) Aust J. Plant Physiol. 4: 143-158]. May be used to detect and quantify AGPs in tissue extracts [Van Holst & Clarke (1985) Anal Biochem. 148: 446-450] and to detect AGPs in crossed-electrophoretic separations [Van Holst & Clarke (1986) Plant Physiol. 80: 786-798].
Gum Arabic an arabinogalactan-protein
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Gum Arabic AGP is used as a reference standard in methods involving detection, quantification and analytical analyses of AGPs from plant tissues.
Monoclonal Antibodies (Murine)
to (1-3)-beta-D-glucan
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No cross-reactivity with (1-4)-beta-D-glucans or (1-3;1-4)-beta-D-glucans [Meikle et al. (1991) Planta 188: 1-8].
to (1-3;1-4)-beta-D-glucan
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No cross-reactivity with (1-3)-beta-D-glucans [Meikle et al. (1994) The Plant Journal 5: 1-9].
to (1-4)-beta-D-mannan and galacto-(1-4)-beta-D-mannan
[Pettolino et al. (2001) Planta 214: 235-242]
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to arabinogalactan-proteins (under licence from University of Leeds, UK)
to heteroxylans (under licence from University of Leeds, UK)
Monoclonal Antibodies (Murine)
to xyloglucans (LM25) (under licence from University of Leeds, UK)
[Pedersen et al. (2012). J. Biological Chemistry 287(47): 39429-39438.]
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to pectic polysaccharides (under licence from University of Leeds, UK)
to extensins (under licence from University of Leeds, UK)
Each of these antibodies can be used with second stage, gold- or fluorochrome-labelled rabbit, anti-mouse antibody for immunohistochemical studies.
(1-3;1-4)-beta-D-glucan Hydrolase from Bacillus subtilis (EC 3.2.1.73)
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Specifically hydrolyses beta-D-glucans containing both (1-3)- and (1-4)-beta-D-glucosidic linkages in linear sequences. Does not hydrolyse (1-4)-beta-D-glucans or (1-3)-beta-D-glucans [Anderson & Stone (1975) FEBS Letters 52: 202-207].
Pachyman [(1-3)-beta-D-glucan] ex Poria cocus
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Useful as a positive control in fluoroescence microscopy studies on callose using the aniline blue fluorochrome or (1-3)-beta-D-glucan specific monoclonal antibody.