Products

Histochemical Reagents

Aniline Blue Fluorochrome for callose and related (1-3)-beta-glucans
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An analytical probe for (1-3)-beta-D-glucans (callose). Highly specific for (1-3)-beta-glucans [Evans et al. (1984) Carbohyd. Polymers 4: 215-230; Stone et al. (1984) Protoplasma 122: 191-195]. May be used for the quantitative determination of callose [Kauss (1989) Plant Physiol. 81: 171-176] and in fluorescence assays for (1-3)-beta-glucan synthase products.

Yariv Reagents for arabinogalactan-proteins (AGPs)
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Specific probes for the detection of AGPs in tissue sections [Anderson et al. (1977) Aust J. Plant Physiol. 4: 143-158]. May be used to detect and quantify AGPs in tissue extracts [Van Holst & Clarke (1985) Anal Biochem. 148: 446-450] and to detect AGPs in crossed-electrophoretic separations [Van Holst & Clarke (1986) Plant Physiol. 80: 786-798].

Gum Arabic an arabinogalactan-protein
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Gum Arabic AGP is used as a reference standard in methods involving detection, quantification and analytical analyses of AGPs from plant tissues.

Immunohistochemical Reagents

Monoclonal Antibodies (Murine)

to (1-3)-beta-D-glucan
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No cross-reactivity with (1-4)-beta-D-glucans or (1-3;1-4)-beta-D-glucans [Meikle et al. (1991) Planta 188: 1-8].

to (1-3;1-4)-beta-D-glucan
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No cross-reactivity with (1-3)-beta-D-glucans [Meikle et al. (1994) The Plant Journal 5: 1-9].

to (1-4)-beta-D-mannan and galacto-(1-4)-beta-D-mannan
[Pettolino et al. (2001) Planta 214: 235-242]
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to arabinogalactan-proteins (under licence from University of Leeds, UK)

to arabinogalactan-proteins, terminal glucuronsyl residues (LM2)
[Smallwood et al. (1996). Planta 198: 452-459.]
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to arabinogalactan-proteins, beta-GlcA-(1-3)-alpha-GalA-(1-2)-Rha epitope (JIM13)
[Knox et al. (1991). Plant Journal 1: 317-326.]
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to heteroxylans (under licence from University of Leeds, UK)

to (1-4)-beta-D-xylan (LM11)
[McCartney et al. (2005). J. Histochemistry & Cytochemistry 53(4): 543-546.]
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to heteroxylan, glucuronoxylan (LM28)
[Cornuault et al. (2015). Planta 242(6): 1321-1334.]
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Immunohistochemical Reagents (continued…)

Monoclonal Antibodies (Murine)

to xyloglucans (LM25) (under licence from University of Leeds, UK)
[Pedersen et al. (2012). J. Biological Chemistry 287(47): 39429-39438.]
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to pectic polysaccharides (under licence from University of Leeds, UK)

to pectic (1-5)-alpha-L-arabinan (LM6)
[Willats et al. (1998). Carbohydrate Research 308(1-2): 149-152.]
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to branched pectic galactan (LM26)
[Torode et al. (2018). Plant Physiology 176(2): 1547-1558.]
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to pectic (1,4)-beta-D-galactan (LM5)
[Jones et al. (1997). Plant Physiology 113(4): 1405-1412.]
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to pectic unesterified homogalacturonan (LM19)
[Verhertbruggen et al. (2009). Carbohydrate Research 344(14): 1858-1862.]
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to pectic methylesterified homogalacturonan (LM20)
[Verhertbruggen et al. (2009). Carbohydrate Research 344(14): 1858-1862.]
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to pectic methylesterified homogalacturonan (JIM7)
[Knox et al. (1990). Planta 181(4): 512-521.]
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to extensins (under licence from University of Leeds, UK)

to extensin (JIM20)
[Smallwood et al. (1994). The Plant Journal 5(2): 237-246.]
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to extensin (LM1)
[Smallwood et al. (1995). Planta 196(3): 510-522]
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Each of these antibodies can be used with second stage, gold- or fluorochrome-labelled rabbit, anti-mouse antibody for immunohistochemical studies.

Enzymes

(1-3;1-4)-beta-D-glucan Hydrolase from Bacillus subtilis (EC 3.2.1.73)
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Specifically hydrolyses beta-D-glucans containing both (1-3)- and (1-4)-beta-D-glucosidic linkages in linear sequences. Does not hydrolyse (1-4)-beta-D-glucans or (1-3)-beta-D-glucans [Anderson & Stone (1975) FEBS Letters 52: 202-207].

Substrates

Pachyman [(1-3)-beta-D-glucan] ex Poria cocus
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Useful as a positive control in fluoroescence microscopy studies on callose using the aniline blue fluorochrome or (1-3)-beta-D-glucan specific monoclonal antibody.

Reference Text

Chemistry and Biology of (1-3)-beta-glucans

By Bruce A Stone and Adrienne E Clarke

Glucans, with the (1-3)-beta-glucosidic linkage as a major feature, are present in most higher plants and many lower plants and microorganisms. They may occur as major structural or storage components or be formed at very specific sites in response to particular developmental events or stimuli. In many cases their functional role is a mystery, in others it is well established. Their distribution and physiological involvement indicates that they are important to fields such as agriculture and biotechnology, and may also have impact in medicine, throught their role in immunology and cancer therapy.

Contents:

Structural characterisation and physical chemistry of:

(1-3)-beta-glucans
Enzyme depolymerising (1-3)-beta-glucans
Biosynthesis of (1-3)-beta-glucans
Prokaryote (1-3)-beta-glucans
Algal (1-3)-beta-glucans
Fungal, yeast and lichen (1-3)-beta-glucans
Chemistry and physiology of higher plants (1-3)-beta-glucans (callose)
(1-3)(1-4)-beta-glucans in higher plants
(1-3)-beta-glucans in plant host-pathogen interactions
(1-3)-beta-glucans and (1-3)-glucan hydrolases in animals
(1-3)-beta-glucans and animal defence mechanisms

References and index

The book is an invaluable reference handbook not only for graduate students and teachers in universities but also for scientists in industrial, agricultural and medical research and develpment laboratories.

Special features of ‘Chemistry and Biology of (1-3)-beta-glucans’ include:

Hard cover & section sewn binding
808 pages
Over 150 tables and illustrations
Exhaustive index of over 50 pages
First published 1992

USD$91 plus postage and handling

…

Chemistry, Biochemistry, and Biology of (1-3)-beta-Glucans and Related Polysaccharides

By Antony Bacic, Geoffrey B. Fincher, Bruce A. Stone

Key Features:

Topics of medical relevance include detailing the glucans’ interactions with the immune system and research for cancer therapy applications
Web resource links allow scientists to explore additional beta glucan research
Separate indexes divided into Species and Subject for enhanced searchability

Description:

The book presents a comprehensive, systematic and authoritative survey of information about a family of chemically related, but functionally diverse, naturally occurring polysaccharides- the (1-3)-glucans. International contributors describe the chemical and physicochemical properties of these glucans and their derivatives and the molecular biological and structural aspects of the enzymes involved in their formation and breakdown. A detailed analysis of their physiological roles in the various biological situations in which they are found will be provided. Additionally, evolutionary relationships among the family of these glucans will be described.

[For more information on this book, click here.]

Pub. Date: August 2009
ISBN-13: 978-0-12-373971-1
Audience: Plant physiologists & cell biologists, enzymologists, polysaccharide chemists, immuno-chemists, immunologists, alternative cancer therapy researchers

Order online today!
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